Bone resorption markers in saliva. Experimental longitudinal study.

PELLEGRINI G; GONZALES CHAVES M; SOMOZA J; FRIEDMAN S; ZENI S

Montrel, Canadá

Congreso; Therty Annual Meeting Of the American Society for Bone and Mineral Research; 2008

ASBMR

Bone resorption markers in saliva. Experimental longitudinal study. Previously, we measured terminal C-telopeptide of collagen type I (CTX) in saliva. The present longitudinal experimental study sought to determine whether salivary concentrations of CTX have the same response as in serum samples under different conditions: normal, increased, and reduced bone remodeling. Thirty rats were ovariectomized (OVX) and 10 rats were SHAM operated. The rats were divided into 4 groups (G) and treated as follows for 45 days: G1 = SHAM+vehicle; G2 = OVX + 8 mg olpadronate (OPD)/100 g body weight (BW); G3 = OVX + 4 mg OPD/100 g BW ;G4 = OVX +vehicle. Saliva and serum CTX (ng/ml) were determined by ELISA (Rat Laps. Osteometer BioTech, Denmark) at baseline (t=0), at t=10, t=20 and t=45 days of surgery. Results (mean±SD): (table 1) There were no differences in salivary CTX levels at baseline. G4 presented the highest salivary CTX levels compared to the other 3 groups throughout the experience (P <0.05). Salivary CTX values remained unchanged in G1 and G2 and decreased in G3 (P <0.01). Salivary CTX levels were similar in G1 and G2 and significantly lower in G3 (p<0.05). Conclusions: Saliva samples responded like serum samples to changes in bone turnover. Then saliva may prove to be a practical and reliable sample to evaluate changes in bone remodeling and to monitor therapy with anticatabolic drugs. Grant M099, UBACyT. BASE LINE T= 10ds T= 20ds T= 45ds saliva Serum saliva Serum saliva Serum saliva serum G1 mean 3,40 39,3 3,93 34,9 3,10 34,4 3,10 33,3 SD 0,95 3,1 1,70 11,3 0,82 3,2 1,17 7,4 G2 mean 3,31 30,1 3,59 42,5 3,17 38,8 3,21 37,6 SD 0,96 9,5 0,88 2,2 1,13 5,7 1,19 8,5 G3 3,56 38,7 3,44 31,8 2,80 33,4 2,77 24,1 0,16 OPD 0,73 2,4 0,59 5,3 0,40 7,8 0,66 7,9 G4 mean 3,30 34,9 4,40 42,6 4,46 43,8 3,93 40,2 SD 0,96 8,5 0,59 8,1 1,08 10,8 0,85 5,9