Rat mandible bone response to atherogenic diet

GAMBA CA, ; MACRI V,; GONZALES CHAVES M, ; ORZUZA R, ; ZAGO V, ; RODRÍGUEZ P, ; MANDALUNIS P, ; ZENI SN, ; FRIEDMAN S

Buenos Aires

Congreso; XXVI Reunión Anual de la AAOMM; 2009

AAOMM

We observed that an atherogenic diet in active growth phase did not induce macroscopic alterations in mandible. However, diminution in bone accretion and alterations in mandible skeletal units could lead to structure alterations. In this study we hypothesized that atherogenic diets could alter bone remodeling in mandible. Male weanling Wistar rats (n=11), randomly divided into control (C) and experimental (E). C received rodent stock diet (pellets) and E, atherogenic diet (pellets+ saturated fatty acids+cholesterol) for 7 weeks (Tf), monitoring anthropometry and diet intake. At Tf, blood (mg/dL) lipid–lipoprotein atherogenic profile: total cholesterol (T-cho), triglycerides (TG), high density lipoprotein-cholesterol (HDL-cho), HDL-non-cholesterol (n- HDL-cho). Dissected hemimandibles, fixed in buffered formalin–PBS 10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal oriented sections of first mandibular molar, stained with hematoxylin– 10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal oriented sections of first mandibular molar, stained with hematoxylin– atherogenic profile: total cholesterol (T-cho), triglycerides (TG), high density lipoprotein-cholesterol (HDL-cho), HDL-non-cholesterol (n- HDL-cho). Dissected hemimandibles, fixed in buffered formalin–PBS 10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal oriented sections of first mandibular molar, stained with hematoxylin– 10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal oriented sections of first mandibular molar, stained with hematoxylin– –lipoprotein atherogenic profile: total cholesterol (T-cho), triglycerides (TG), high density lipoprotein-cholesterol (HDL-cho), HDL-non-cholesterol (n- HDL-cho). Dissected hemimandibles, fixed in buffered formalin–PBS 10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal oriented sections of first mandibular molar, stained with hematoxylin– 10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal oriented sections of first mandibular molar, stained with hematoxylin– –PBS 10%, decalcified in EDTA (pH 7.2), embedded in paraffin for mesio-distal oriented sections of first mandibular molar, stained with hematoxylin–– eosin, where histomorphometric bone volume (BV) was measured. Statistics: Student´s t test and Pearson. Results (mean±SD) at Tf: no anthropometric or intake differences (p>0.05); In E, lipid profile showed significant increases in T-cho (96.2±8.9 vs 56.4±2.5: p<0.0001), n-HDL-cho (57.8±6.9 vs 21.0±3.5: p<0.0001), but the opposite in TG (76.5± 16.4 vs 163.2±47.4: p=0.002). BV in E was significantly reduced (35.9±6.8 vs 47.7±7.4: p=0.022). T-cho vs BV correlation was found highly significant (p=0.0095). These results suggest that anatherogenic diet alters bone remodeling of interradicular bone in mandible, accounted by diminished BV. UBACyT O008. anthropometric or intake differences (p>0.05); In E, lipid profile showed significant increases in T-cho (96.2±8.9 vs 56.4±2.5: p<0.0001), n-HDL-cho (57.8±6.9 vs 21.0±3.5: p<0.0001), but the opposite in TG (76.5± 16.4 vs 163.2±47.4: p=0.002). BV in E was significantly reduced (35.9±6.8 vs 47.7±7.4: p=0.022). T-cho vs BV correlation was found highly significant (p=0.0095). These results suggest that anatherogenic diet alters bone remodeling of interradicular bone in mandible, accounted by diminished BV. UBACyT O008. t test and Pearson. Results (mean±SD) at Tf: no anthropometric or intake differences (p>0.05); In E, lipid profile showed significant increases in T-cho (96.2±8.9 vs 56.4±2.5: p<0.0001), n-HDL-cho (57.8±6.9 vs 21.0±3.5: p<0.0001), but the opposite in TG (76.5± 16.4 vs 163.2±47.4: p=0.002). BV in E was significantly reduced (35.9±6.8 vs 47.7±7.4: p=0.022). T-cho vs BV correlation was found highly significant (p=0.0095). These results suggest that anatherogenic diet alters bone remodeling of interradicular bone in mandible, accounted by diminished BV. UBACyT O008.