Recovery in serum 25OHD by dietary vitamin D2 or D3 in an experimental model of vitamin D insufficiency and established osteopenia.

GONZALES CHAVES MMS, . ; MAROTTE C, ; PELLEGRINI GG, ; FRIEDMAN SM ; ZENI SN

Buenos Aires

Congreso; XXVI Reunión Anual de la AAOMM.; 2009

AAOMM

Previous studies suggested that vitamin (vit) D3 is almost twice more potent than vitD2 in raising 25OHD levels (J Clin Met Endoc3 is almost twice more potent than vitD2 in raising 25OHD levels (J Clin Met Endoc2 in raising 25OHD levels (J Clin Met Endoc 93:3015, 2008). To confirm experimentally this suggestion we performed a study using our model of vitD insufficiency and established osteopenia (Bone 39: 837–844, 2006). Female Wistar rats (200±50 g) were ovariectomized (OVX) (n=32) or shamoperated (SHAM) (n=32). During 15 days after surgery all rats fed a diet containing 15% protein, 200 IU% vitD, and 0.6% calcium. For an additional period of 45 days a diet without vitD (0 IU%) were supplied to obtain vitD insufficiency. After 105 days rats were divided into 4 groups that fed a diet containing 200 UI% of vitD2 (OVX+vitD2 and SHAM+vitD2) or vitD3: (OVX+vitD3 and SHAM+vitD3). Basal 25OHD levels were randomly obtained in 9 rats. At 60, 85, and 105 days of experience serum 25(OH)D (ng/ml) was determined in each group by employing the methodology considered to assess both 25OHD3 and 25OHD2 (125I RIA Kit, DiaSorin, Stillwater, MN, USA). The results of 25OHD were (x±ES): basal 19.2 ± 0.3: OVX+D2Bone 39: 837–844, 2006). Female Wistar rats (200±50 g) were ovariectomized (OVX) (n=32) or shamoperated (SHAM) (n=32). During 15 days after surgery all rats fed a diet containing 15% protein, 200 IU% vitD, and 0.6% calcium. For an additional period of 45 days a diet without vitD (0 IU%) were supplied to obtain vitD insufficiency. After 105 days rats were divided into 4 groups that fed a diet containing 200 UI% of vitD2 (OVX+vitD2 and SHAM+vitD2) or vitD3: (OVX+vitD3 and SHAM+vitD3). Basal 25OHD levels were randomly obtained in 9 rats. At 60, 85, and 105 days of experience serum 25(OH)D (ng/ml) was determined in each group by employing the methodology considered to assess both 25OHD3 and 25OHD2 (125I RIA Kit, DiaSorin, Stillwater, MN, USA). The results of 25OHD were (x±ES): basal 19.2 ± 0.3: OVX+D2n=32) or shamoperated (SHAM) (n=32). During 15 days after surgery all rats fed a diet containing 15% protein, 200 IU% vitD, and 0.6% calcium. For an additional period of 45 days a diet without vitD (0 IU%) were supplied to obtain vitD insufficiency. After 105 days rats were divided into 4 groups that fed a diet containing 200 UI% of vitD2 (OVX+vitD2 and SHAM+vitD2) or vitD3: (OVX+vitD3 and SHAM+vitD3). Basal 25OHD levels were randomly obtained in 9 rats. At 60, 85, and 105 days of experience serum 25(OH)D (ng/ml) was determined in each group by employing the methodology considered to assess both 25OHD3 and 25OHD2 (125I RIA Kit, DiaSorin, Stillwater, MN, USA). The results of 25OHD were (x±ES): basal 19.2 ± 0.3: OVX+D2n=32). During 15 days after surgery all rats fed a diet containing 15% protein, 200 IU% vitD, and 0.6% calcium. For an additional period of 45 days a diet without vitD (0 IU%) were supplied to obtain vitD insufficiency. After 105 days rats were divided into 4 groups that fed a diet containing 200 UI% of vitD2 (OVX+vitD2 and SHAM+vitD2) or vitD3: (OVX+vitD3 and SHAM+vitD3). Basal 25OHD levels were randomly obtained in 9 rats. At 60, 85, and 105 days of experience serum 25(OH)D (ng/ml) was determined in each group by employing the methodology considered to assess both 25OHD3 and 25OHD2 (125I RIA Kit, DiaSorin, Stillwater, MN, USA). The results of 25OHD were (x±ES): basal 19.2 ± 0.3: OVX+D22 (OVX+vitD2 and SHAM+vitD2) or vitD3: (OVX+vitD3 and SHAM+vitD3). Basal 25OHD levels were randomly obtained in 9 rats. At 60, 85, and 105 days of experience serum 25(OH)D (ng/ml) was determined in each group by employing the methodology considered to assess both 25OHD3 and 25OHD2 (125I RIA Kit, DiaSorin, Stillwater, MN, USA). The results of 25OHD were (x±ES): basal 19.2 ± 0.3: OVX+D22) or vitD3: (OVX+vitD3 and SHAM+vitD3). Basal 25OHD levels were randomly obtained in 9 rats. At 60, 85, and 105 days of experience serum 25(OH)D (ng/ml) was determined in each group by employing the methodology considered to assess both 25OHD3 and 25OHD2 (125I RIA Kit, DiaSorin, Stillwater, MN, USA). The results of 25OHD were (x±ES): basal 19.2 ± 0.3: OVX+D23 and 25OHD2 (125I RIA Kit, DiaSorin, Stillwater, MN, USA). The results of 25OHD were (x±ES): basal 19.2 ± 0.3: OVX+D2x±ES): basal 19.2 ± 0.3: OVX+D2 T:60=5.0±0.9a, T:85=16.6±1.5b, T:105=15.5±0.3b; OVX+D33 T:60=5.0±0.6a, T:85=15.1±0.6b, T:105=15.9±1.0b; SHAM+D22 T:60=4.8±0.5a, T:85=14.7±1.4b, T:105=15.5±0.9b; SHAM+D33 T:60=6.0±1.2a, T:85=16.6±2.1b, T:105=18.0±1.3b. Different letters indicate p< 0.05. Results indicate no differences in 25(OH)D levels because of feeding vitD2 or vitD3. Conclusions: Under our experimental conditions, vitD2 and vitD3 supplied daily had the same power to maintain and to increase 25(OH)D levels, in both estrogen conditions. PIP 6483.2 or vitD3. Conclusions: Under our experimental conditions, vitD2 and vitD3 supplied daily had the same power to maintain and to increase 25(OH)D levels, in both estrogen conditions. PIP 6483.2 and vitD3 supplied daily had the same power to maintain and to increase 25(OH)D levels, in both estrogen conditions. PIP 6483.