INVESTIGADORES
CASTELLANOS Lucia Ines
congresos y reuniones científicas
Título:
Synthesis of quorum sensing signals by diazotrophs from the rhizosphere of strawberry plants
Autor/es:
NIETO PEÑALVER CG; BICHARA L; CASTELLANOS DE FIGUEROA, LUCÍA INÉS; DÍAZ RICCI JC
Lugar:
Puerto Madryn, Chubut, Argentina
Reunión:
Congreso; 46th Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
In the rhizosphere, interactions occur with
both pathogenic and beneficial microorganisms. The latter group can stimulate
plant growth by mean of phytohormone production, direct antagonism of pathogens
or induction of systemic resistance in the plant. Quorum sensing (QS) systems
from non symbiotic diazotrophic bacteria may play a role in the interactions with
strawberry plants and with other organisms. The objective of this work was the
characterization of N-acylhomoserine lactones (N-AHLs) produced
by free living nitrogen fixing bacteria associated to roots of Fragaria
ananassa Duch. cv. Camarosa. Strains were isolated and purified in nitrogen
free semisolid media. Extracts were prepared with acidified ethyl acetate and
samples were analyzed by TLC. Chromobacterium violaceum Vir07, Agrobacterium
tumefaciens NTL4 (pCF218 pCF372) and Pseudomonas putida F117
(pKR-C12) were utilized as biosensor strains for the detection of QS molecules.
Nonsymbiotic diazotrophic bacteria producing QS molecules could be isolated
from roots of strawberry plants. Production of short chain N-AHLs was
detected in all strains. Only one strain produced long chain N-AHLs. Although
biosynthesis of QS molecules is not widely distributed in diazotrophic strains
from strawberry roots,secretion of short chain QS molecules seem to play a key
role as signaling molecules.