INVESTIGADORES
CASTELLANOS Lucia Ines
congresos y reuniones científicas
Título:
Culture medium factorial design optimization for fibrinolytic enzymes production by Bionectria sp
Autor/es:
ARNAU, GONZALO; ROVATI JOSE IGNACIO; CASTELLANOS DE FIGUEROA, LUCÍA INÉS; FARIÑA J.I,
Lugar:
Puerto Madryn, Chubut, Argentina
Reunión:
Congreso; 46th Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
Thrombotic
diseases can be clinically treated with fibrinolytic enzymes and many attempts
have been made at laboratory level to increase fibrinolytic enzymes production
from microbial sources and to reduce the process cost, including culture medium
design, optimization of environmental conditions, and over expression with
genetically modified strains. In this contribution we present the optimization
of culture medium composition and incubation temperature for fibrinolytic
enzyme production by Bionectria sp., a selected fungal strain from Las Yungas
(Tucumán). Optimization was carried out at Erlenmeyer scale (100-mL working
volume) via factorial design methodology. All trials included a common mineral
base (%, w/v: NaCl 0.2, KH2PO4 0.05, MgSO4.7H2O 0.05). According to four
factorial designs it could be demonstrated the convenience of using soy peptone
as N-source, glucose as C-source, and the possibility to eliminate starch, meat
peptone and meat extract from original medium composition, whilst 25°C was selected as the
optimal incubation temperature. Results showed that culture medium could be
successfully optimized by factorial design, achieving a reduction in the
production process costs by means of a decrease in culture medium components,
the improvement in culture broth rheology, mycelial morphology and mass/energy
transfer, and the subsequent two-fold enhancement in productivity.