Early postnatal exposure to xenoestrogens alters the expression of molecules involved inthe postnatal differentiation of the oviduct ofthe broad-snouted caiman (Caiman latirostris)

TAVALIER, YE; GALOPPO GH; MORA SC; LUQUE EH; MUÑOZ-DE-TORO M

Congreso; Reunión Anual SAIC ? SAFE ? SAB ? SAP 2019; 2019

The differentiation of the oviduct of Caiman latirostris is completed postnatally and is characterized bytemporal-spatial patterns of histofunctional changes. We have shown that the exposure to Bisphenol A(BPA) or 17β-Estradiol (E2) at critical stages of embryo development affects oviduct postnataldifferentiation, leading to a precocious adenogenesis. Adenogenesis is regulated by the Wnt signalingpathway in many vertebrates. The aim of this study was to determine the molecular mechanism behindaltered adenogenesis. We established the ontogeny and evaluated the effects of postnatal exposure toBPA or E2 on the expression of molecules of the Wnt signaling pathway: Wnt-7a, Wnt-5a, β-catenin andFoxA2. Archived paraffin-embedded samples were used to establish ontogenies. To assess the effect ofxenoestrogens,early postnatal caimans were treated with E2 (0.014 or 1.4 ppm) or BPA (1.4 or 140 ppm).Protein expression was assessed by IHC. ANOVA or Kruskal-Wallis was performed to obtain the overallsignificance, followed by Dunnett?s or Dunn?s test, respectively; p