Perinatal exposure to a glyphosate based herbicide causes implantation failures and transgenerational induction of congenital anomalies in rats.

GUILLERMINA PACINI, ; JORGELINA G. VARAYOUD,; MA. MERCEDES MILESI, JORGELINA VARAYOUD, RAMIRO ALARCÓN, MÓNICA MUÑOZ-DE-TORO, ENRIQUE LUQUE; ENRIQUE H. LUQUE,; MA. MERCEDES MILESI.

Mar del Plata, Argentina

Otro; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clinica.; 2016

)lyphosate based herbicides )$H are extensively used foragricultural purposes all over the Yorld, Yhich is closely associatedYith a constant increase in the use of transgenic glyphosateresistant soybean singlecropping. 4ecently, Ye shoYed that abrief exposure to a )$H, administered subcutaneously during thefirst postnatal YeeM alters uterine development in prepubertal rats,and induces postimplantation embryo loss at adulthood. +n thepresent study Ye evaluate if an oral administration of )$H duringthe perinatal period gestation and lactation affects female fertilityand/or induces transgenerational effects on prenatal developmentof their progeny. 2regnant rats F Yere orally exposed to mg )$H/-g/day through food, from gestational day )& untilYeaning postnatal day , 20&. The body Yeight gain andthe vaginal canalopening of the F females Yere evaluated. 1n20&, F females Yere submitted to a fertility test to evaluate thepregnancy rates, and on )&, the number of corpora lutea CLsand the implantation and resorption sites. To determine transgenerationaleffects on the F offspring development, Ye evaluate thefetal Yeight, length and morphology, and the placental Yeight.)$H exposure did not alter the body Yeight gain of the F femalesYith age, but led to early onset of vaginal opening, indicating earlypuberty. Although all )$Htreated F females resulted pregnant,a decreased number of implanted embryos Yere detected. /oreover,F offspring exhibited a delayed groYth, evidenced by loYerfetal Yeight and length. A higher placental Yeight Yas detectedin the )$H group. Surprisingly, structural congenital anomalies,such as, conjoined fetuses and abnormally developed limbs Yeredetected in the F offspring. 9e concluded that perinatal exposureto a )$H induced female subfertility by decreasing the numberof implanted embryos, and caused transgenerational induction ofcongenital anomalies.