Advanced Caiman Latirostris Oviductal Maturation After Postnatal Exposure To Xenoestrogens.

GALOPPO G; CANESINI G; DURANDO M; LUQUE E.H.; MUÑOZ-DE-TORO M.

Chascomús, Buenos Aires

Jornada; XVI JORNADAS DE LA SOCIEDAD ARGENTINA DE BIOLOGÍA "IMPACTO ANTRÓPICO SOBRE LA FISIOLOGÍA DE LOS ORGANISMOS"; 2014

Caiman latirostris oviductal maturation is a postnatal event characterized by changes in luminal epithelium and in the subepithelial stroma. We described that; adenogenesis follows gradual collagen disorganization and increased expression of smooth-muscle proteins. The aim of our study was to assess whether early postnatal exposure to 17β-estradiol (E2) or to bisphenol A (BPA) alters maturation-related parameters. Two injections (s.c), 7 days apart from each other, of E2 (1.4 and 0.014ppm), BPA (140 and 1.4ppm) or vehicle were administered to 30 day old female caimans. Seven days after the last injection, oviducts were paraffin-processed. Collagen organization was assessed by picrosirius-polarization method. Desmin and smooth-muscle α-actin (α-SMA) expression were evaluated by immunohistochemistry. All treatments increased the area occupied by poorly organized collagen. Low doses of both E2 and BPA increased the proportion of subepithelial area occupied by desmin and α-SMA. Adenogenesis was absent. Postnatal exposure to xenoestrogens advanced the oviductal maturation. These changes could impair the reproductive performance of sexually mature caimans.