INVESTIGADORES
PANIEGO Norma Beatriz
capítulos de libros
Título:
Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology
Autor/es:
AGUIRRE, NATALIA C.; FILIPPI, C. V.; VERA P; PUEBLA A; ZAINA, GIUSI; LIA V; MARCUCCI POLTRI, SUSANA N.; PANIEGO, N. B.
Libro:
Plant Genotyping: Methods and Protocols
Editorial:
Springer
Referencias:
Lugar: New York; Año: 2023; p. 37 - 57
Resumen:
Double digest restriction-site associated DNA sequencing (ddRADseq) technology combines genome reduced representation by digestion with two restriction enzymes and next generation sequencing (NGS) to obtain thousands of markers (SNP, SSR, and InDels) and genotype tens to hundreds of samples simultaneously. In this chapter, we describe a 96-plex derived ddRADseq protocol that can be set up to obtain different depth of coverage per locus and can be exploited to model and non-model plant species.
