MODIFICATION ON LIPID KINASE ACTIVITIES INDUCED BY PHENOL TREATMENT IN TRANSGENIC TOBACCO HAIRY ROOTS

LG SOSA ALDERETE; RACAGNI, G; AGOSTINI E; MEDINA MI

Puerto Iguazu, Misiones

Conferencia; 56 th International Conference on the Bioscience of Lipids. 22-26 september 2015; 2015

Lipids

Plants in their natural habitats are exposed to several environmental stressors affecting their survival. Furthermore, as a consequence of antropogenic activity, an increased release of different pollutants, such as phenol, to the environment, has generated another stressful situation to the development and growth of the plants. In this work, we studied the effects of phenol treatment on the biosynthesis of several phosphoinositides (PI) and phospholipids (PL) such as phosphatidylinositol monophosphate (PIP), phosphatidylinositol bisphosphate (PIP2) and lisophosphatidic acid (LPA), diacylglycerolpyrophosphate (DGPP) and phosphatidic acid (PA), respectively. These compounds are synthesized by specific PI and PL kinases present in the phosphoinositide pathway and play key role as signal molecules in response to stressful conditions. The study was carried out using tobacco hairy roots (HRs), double transgenic (DT) for two peroxidase genes and wild type (WT) ones. In both HRs, phenol treatment significantly increased the biosynthesis of these compounds. However, these changes were different between both HRs. WT HRs, the PA formation was 3 fold higher than in the control. The levels of PIP2 and PIP were 2-fold higher than those of the controls. The levels of LPA and DGPP fractions detected after phenol exposure increased by 100 and 50% respectively. Regarding DT HRs, the levels of PIP2, PIP, LPA/DGPP and PA were 2-fold higher than those detected in the controls. These results suggest, for first time, that phosphoinositide pathway, might be an important mechanism of plant tissues in response to phenol treatment.