TERATOGENIC EFFECTS OF PHENOL IN EMBRYOS OF BUFO ARENARUM

PAISIO C, AGOSTINI E, GONZÁLEZ P, RIVAROLA V, BERTUZZI M.

Huerta Grande (Cba)

Congreso; I Reunión Conjunta de Sociedades de Biología de la República Argentina; 2007

Sociedades de Biología de la República Argentina

Phenol and its derivatives are thoroughly used in the industry and they have a high potential toxicity to animals and plants. In previous studies we demonstrated that phenol’s solutions in concentrations from 100 to 250 mg/l produced mortality in metamorphic embryos of Bufo arenarum. The aim of the present work was to determine the teratogenic effects of phenol in premetamorfic embryos of this amphibians. The embryos were obtained by in vitro fecundation and they were exposed to solutions of phenol of 0 (control) 25, 50, 100, 150 and 200 mg/l for 4 days, to analyze malformations and behavior changes. The observed effects were: alterations in blastulae and gastrulae, irregular forms, reduction of the body, edema, absence of eyes, damages in the blastoporo, absence of the gills, deviations of the axis, necrosis, circulate swim, cutaneous alterations, and acephalism. In the concentrations of 25 and 50 mg/l prevailed the malformations in blastulae and gastrulae, deviations of the axis, damages of the blastoporo and acephalism while in 100 mg/l solutions, damages in the blastoporo, bend and reduction of the axis and acephalism were observed. In 150 mg/l, the most frequent alterations were the bend of the axis, widespread edema and irregular forms. The concentration of 200 mg/l produced 100% of mortality. The results showed that phenol produced teratogenic effects from the smallest concentration used and that the premetamorfic stages possess more sensibility than the metamorphic stages.