INVESTIGADORES
AGOSTINI Elizabeth
artículos
Título:
Phytoremediation of 2,4-dichlorophenol by Brassica napus hairy root cultures
Autor/es:
ELIZABETH AGOSTINI; MARIA S. CONIGLIO; SILVIA MILRAD; HORACIO TIGIER; ANA M GIULIETTI
Revista:
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
Editorial:
PORTLAND PRESS LTD
Referencias:
Lugar: Gran Bretaña; Año: 2003 vol. 37 p. 139 - 144
ISSN:
0885-4513
Resumen:
We have obtained hairy root cultures of Brassica napusBrassica napus
with high biomass and genetic stability which produce
peroxidases, enzymes involved in biodegradation processes.
In this work, these hairy root cultures were used
to study the removal of 2,4-dichlorophenol (2,4-DCP), a
common contaminant in industrial effluents that is
highly toxic for human and aquatic life. The optimum
conditions to obtain high efficiency in the removal
process were established. Roots were able to remove
2,4-DCP from aqueous solutions containing 100
1000 mg/l, in the presence ofH2O2 concentrations ranging
from 5 to 10 mM. After a short period of incubation
(15 min), high removal efficiencies were achieved
(9194%) and maximal removal, of approx. 9798%,
was obtained with 1 h of reaction. High removal
efficiencies (9395%) were observed in a broad pH
range (pH 39), reaching 9899% in the range pH 48.
Moreover, roots could be re-used, almost for six
consecutive cycles, to remove 2,4-DCP. The oxidation
catalysed by peroxidases would be the main mechanism
involved in this process. The results suggest that these
cultures could be useful tools for phytoremediation2O2 concentrations ranging
from 5 to 10 mM. After a short period of incubation
(15 min), high removal efficiencies were achieved
(9194%) and maximal removal, of approx. 9798%,
was obtained with 1 h of reaction. High removal
efficiencies (9395%) were observed in a broad pH
range (pH 39), reaching 9899% in the range pH 48.
Moreover, roots could be re-used, almost for six
consecutive cycles, to remove 2,4-DCP. The oxidation
catalysed by peroxidases would be the main mechanism
involved in this process. The results suggest that these
cultures could be useful tools for phytoremediation