INVESTIGADORES
THOMAS Andres Hector
artículos
Título:
First steps in the photochemistry of folate in alkaline medium
Autor/es:
ANDRÉS H. THOMAS; FERNANDO S. GARCÍA EINSCHLAG; MARIO L. FÉLIZ; ALBERTO L. CAPPARELLI
Revista:
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Editorial:
Elsevier
Referencias:
Año: 1998 vol. 116 p. 187 - 190
ISSN:
1010-6030
Resumen:
A laser flash photolysis study is reported on alkaline solutions of folic acid in absence of dioxygen. Spectra of the intermediates and their
decays are analysed. A biexponential fit of the experimental decay can be observed in the resolution time window of the technique. Intermediates
spectra obtained in laser flash photolysis were compared with those generated in pulse radiolysis experiments performed on these solutions
under oxidative and reductive conditions. Conventional flash photolysis were also applied in this studies in absence of 02. In this case two
processes are detected, one in the millisecond time window and a very slow process which can be followed by normal spectrophotometry
after flashing solutions. The experimental behaviour of this system is compared with those reported on pterines derivatives, where absorption
changes induced by flash photolysis have been attributed to triplet excited state. The evidence presented here shows that folate suffers an early
photochemical transformation. Radicals generation as a consequence of the absorption of radiation is postulated in order to give a plausible
explanation of the experimental results.