Fluorescence of pterin, 6-formylpterin, 6-carboxypterin and folic acid in aqueous solutions: pH effects

ANDR¨¦S H. THOMAS; CAROLINA LORENTE; ALBERTO L. CAPPARELLI; MEGH RAJ POKHREL; ANDR¨¦ M. BRAUN; ESTHER OLIVEROS

Photochemical and Photobiological Sciences

Royal Society of Chemistry

Año: 2002 vol. 1 p. 421 - 426

1474-905X

Steady-state and time-resolved studies have been performed on four compounds of the pterin family (pterin, 6-carboxypterin, 6-formylpterin and folic acid) in aqueous solution, using the single photon counting technique. The fluorescence characteristics (spectra, quantum yields, lifetimes) of these compounds and their dependence on the pH have been investigated. Most pterins can exist in two acid¨Cbase forms over the pH range between 3 and 13. Emission spectra and excitation spectra were obtained for both forms of each compound studied. Fluorescence quantum yields (¦µF) in acidic and basic media were measured. The ¦µF of folic acid (<0.005 in both media) is very low compared to those of pterin (0.27 in basic media and 0.33 in acidic media), 6-carboxypterin (0.18 in basic media and 0.28 in acidic media) and 6-formylpterin (0.07 in basic media and 0.12 in acidic media). The variation in integrated fluorescence intensity and fluorescence lifetimes (¦ÓF) was analysed as a function of pH. Dynamic quenching by OH was observed and the corresponding bimolecular rate constants for quenching of fluorescence (kq) were calculated. The reported values for kq (M1 s1) are 3.6 ¡Á 109, 1.9 ¡Á 109and 1.1 ¡Á 1010 M1 s1 for pterin, 6-carboxypterin and 6-formylpterin, respectively.uorescence characteristics (spectra, quantum yields, lifetimes) of these compounds and their dependence on the pH have been investigated. Most pterins can exist in two acid¨Cbase forms over the pH range between 3 and 13. Emission spectra and excitation spectra were obtained for both forms of each compound studied. Fluorescence quantum yields (¦µF) in acidic and basic media were measured. The ¦µF of folic acid (<0.005 in both media) is very low compared to those of pterin (0.27 in basic media and 0.33 in acidic media), 6-carboxypterin (0.18 in basic media and 0.28 in acidic media) and 6-formylpterin (0.07 in basic media and 0.12 in acidic media). The variation in integrated fluorescence intensity and fluorescence lifetimes (¦ÓF) was analysed as a function of pH. Dynamic quenching by OH was observed and the corresponding bimolecular rate constants for quenching of fluorescence (kq) were calculated. The reported values for kq (M1 s1) are 3.6 ¡Á 109, 1.9 ¡Á 109and 1.1 ¡Á 1010 M1 s1 for pterin, 6-carboxypterin and 6-formylpterin, respectively.¦µF) in acidic and basic media were measured. The ¦µF of folic acid (<0.005 in both media) is very low compared to those of pterin (0.27 in basic media and 0.33 in acidic media), 6-carboxypterin (0.18 in basic media and 0.28 in acidic media) and 6-formylpterin (0.07 in basic media and 0.12 in acidic media). The variation in integrated fluorescence intensity and fluorescence lifetimes (¦ÓF) was analysed as a function of pH. Dynamic quenching by OH was observed and the corresponding bimolecular rate constants for quenching of fluorescence (kq) were calculated. The reported values for kq (M1 s1) are 3.6 ¡Á 109, 1.9 ¡Á 109and 1.1 ¡Á 1010 M1 s1 for pterin, 6-carboxypterin and 6-formylpterin, respectively.fluorescence intensity and fluorescence lifetimes (¦ÓF) was analysed as a function of pH. Dynamic quenching by OH was observed and the corresponding bimolecular rate constants for quenching of fluorescence (kq) were calculated. The reported values for kq (M1 s1) are 3.6 ¡Á 109, 1.9 ¡Á 109and 1.1 ¡Á 1010 M1 s1 for pterin, 6-carboxypterin and 6-formylpterin, respectively.fluorescence lifetimes (¦ÓF) was analysed as a function of pH. Dynamic quenching by OH was observed and the corresponding bimolecular rate constants for quenching of fluorescence (kq) were calculated. The reported values for kq (M1 s1) are 3.6 ¡Á 109, 1.9 ¡Á 109and 1.1 ¡Á 1010 M1 s1 for pterin, 6-carboxypterin and 6-formylpterin, respectively.fluorescence (kq) were calculated. The reported values for kq (M1 s1) are 3.6 ¡Á 109, 1.9 ¡Á 109and 1.1 ¡Á 1010 M1 s1 for pterin, 6-carboxypterin and 6-formylpterin, respectively.kq (M1 s1) are 3.6 ¡Á 109, 1.9 ¡Á 109and 1.1 ¡Á 1010 M1 s1 for pterin, 6-carboxypterin and 6-formylpterin, respectively.