Influence of gelling agents on Ilex paraguariensis tissue culture

SANSBERRO, P.; REY, H; LUNA, C.; MROGINSKI, L.

Tampere, Findlandia

Congreso; The 4th International Symposium on in vitro Culture and Horticultural Breeding.; 2000

International Society for Horticultural Science (ISHS)

    The effect of six gelling agents (Agar A-1296 ; Agar A-9799 ; Agar A-7921 ; Agarose A-0169 ; Agargel A-3301 and Phytagel P-8169. All of them from Sigma Chemical Co.) on different in vitro tissue culture systems of Ilex paraguariensis  was studied. Interactions were found between type of agar and plant regeneration. Somatic embryogenesis by in vitro culture of immature embryos was not affected by the type of agar. However, Agargel promoted  induction of callus from leaves, while Phytagel was the best gelling agent for callus growth . Although, the in vitro establishment of nodal segments was not affected by the type of gelling agent, the multiplication phase  as well as the rooting of regenerated shoots were greatly increased by Phytagel.  Phytagel was also found to be the more adequate gelling agent for in vitro maturation and germination of immature zygotic embryos The effect of six gelling agents (Agar A-1296 ; Agar A-9799 ; Agar A-7921 ; Agarose A-0169 ; Agargel A-3301 and Phytagel P-8169. All of them from Sigma Chemical Co.) on different in vitro tissue culture systems of Ilex paraguariensis  was studied. Interactions were found between type of agar and plant regeneration. Somatic embryogenesis by in vitro culture of immature embryos was not affected by the type of agar. However, Agargel promoted  induction of callus from leaves, while Phytagel was the best gelling agent for callus growth . Although, the in vitro establishment of nodal segments was not affected by the type of gelling agent, the multiplication phase  as well as the rooting of regenerated shoots were greatly increased by Phytagel.  Phytagel was also found to be the more adequate gelling agent for in vitro maturation and germination of immature zygotic embryos The effect of six gelling agents (Agar A-1296 ; Agar A-9799 ; Agar A-7921 ; Agarose A-0169 ; Agargel A-3301 and Phytagel P-8169. All of them from Sigma Chemical Co.) on different in vitro tissue culture systems of Ilex paraguariensis  was studied. Interactions were found between type of agar and plant regeneration. Somatic embryogenesis by in vitro culture of immature embryos was not affected by the type of agar. However, Agargel promoted  induction of callus from leaves, while Phytagel was the best gelling agent for callus growth . Although, the in vitro establishment of nodal segments was not affected by the type of gelling agent, the multiplication phase  as well as the rooting of regenerated shoots were greatly increased by Phytagel.  Phytagel was also found to be the more adequate gelling agent for in vitro maturation and germination of immature zygotic embryos