ANALYSIS OF T-2 AND HT-2 TOXINS IN SOYBEAN AND SOY MEAL USINGIMMUNOAFFINITY CLEAN-UP AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

BARROS G.G.; GARCIA D.; OVIEDO M.S; RAMIREZ M.L.; TORRES A.M.; CHULZE S.N.

Merida, Mexico

Congreso; IV Congreso Latinoamericano de Micotoxicología; 2010

Sociedad Latinoamericana de Micotoxicologia

Soybean (Glycine max L. Merr.) is the main source of protein worldwide used both as food and feedstuffs. Hygienic safety of soybean and by-products depends on fungal contamination among other microorganisms. Fusarium rot of soybeans is described in the literature and different Fusarium species have been isolated from this commodity (Pitt & Hocking 1999). Fusarium species are known to produce a broad spectrum of toxins including type A- and B- trichothecenes. Among A-type trichothecenes, T-2 and HT-2 toxins are relevant mycotoxins produced mainly by F. sporotrichioides, F. poae, F. equiseti and F. acuminatum (Leslie and Summerell, 2006). In a previous study Fusarium species potential mycotoxins producers have been isolated from soybean in Argentina, however limited information about the occurrence of Fusarium mycotoxins in soybean and by-products is available. Aim: to evaluate an HPLC analysis coupled with immunoaffinity column clean-up previously described by Visconti et al. (2005) for simultaneous determination of T-2 and HT-2 in cereal  grains to be applied in soybean and soy meal samples. to evaluate an HPLC analysis coupled with immunoaffinity column clean-up previously described by Visconti et al. (2005) for simultaneous determination of T-2 and HT-2 in cereal  grains to be applied in soybean and soy meal samples.