Survey of T-2 and HT-2 toxins in soybean and soy meal from Argentina using immunoaffinity clean-up and high performance liquid chromatography

BARROS G.G.; GARCIA D.; OVIEDO M.S; RAMIREZ M.L.; TORRES A.M.; LATTANZIO V.; PASCALE M.; CHULZE S.N.

World Mycotoxin Journal

Wageningen Academic Press

Lugar: Wagenigen; Año: 2011 vol. 4 p. 189 - 197

1875-0710

This work evaluated a method for simultaneous determination of T-2 and HT-2 in soybean and soy meal by using HPLC analysis coupled with an immunoaffinity column clean-up. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of recovery test with spiked soybean and soy meal samples. Mean recoveries for T-2 within the spiking range 125-500 µg/kg, were 90.9 and 81.3% for soybean and soy meal, respectively with a within-laboratory relative standard deviation < 10%. Analysis of samples spiked with HT-2 in the same range gave a mean recovery of 70.2 and 77.5% for soybean and soy meal, respectively, with relative standard deviations < 12%. The limit of detection (LOD) for the method was 25 µg/kg for T-2 and HT-2, based on a signal-to-noise ratio 3:1 and the limit of quantification (LOQ) was established as three times the detection limit.