In vitro control of growth and fumonisin production by Fusarium verticillioides and F. proliferatum using antioxidants under different water availability and temperature regimes

ETCHEVERRY M. G.; TORRES A.M.; RAMIREZ M.L.; CHULZE S.N.; MAGAN N

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Año: 2002 vol. 92 p. 624 - 632

1364-5072

Aims: To examine the effect of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), trihydroxybutyrophenone and propylparaben (PP) (at concentrations of 1±20 mmol l)1) on growth of and fumonisin production by Argentinian strains of Fusarium verticillioides and F. proliferatum. verticillioides and F. proliferatum. (BHT), trihydroxybutyrophenone and propylparaben (PP) (at concentrations of 1±20 mmol l)1) on growth of and fumonisin production by Argentinian strains of Fusarium verticillioides and F. proliferatum. verticillioides and F. proliferatum. To examine the effect of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), trihydroxybutyrophenone and propylparaben (PP) (at concentrations of 1±20 mmol l)1) on growth of and fumonisin production by Argentinian strains of Fusarium verticillioides and F. proliferatum. verticillioides and F. proliferatum. )1) on growth of and fumonisin production by Argentinian strains of Fusarium verticillioides and F. proliferatum.and F. proliferatum. Methods and Results: Studies on lag phases prior to growth, relative growth rates and fumonisin concentrations were carried out in vitro in relation to water activity (0á995±0á93 aw) and temperature (18 and 25°C) on a maize meal agar. Overall, PP was the antioxidant which was most effective at inhibiting strains of both species. The lag phase prior to growth and growth rates were signi®cantly decreased by PP and BHA at 10 and 20 mmol l)1, regardless of the temperature or aw level tested. Total fumonisin production was higher at 0á98 aw and decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 01. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both the temperature or aw level tested. Total fumonisin production was higher at 0á98 aw and decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both was most effective at inhibiting strains of both species. The lag phase prior to growth and growth rates were signi®cantly decreased by PP and BHA at 10 and 20 mmol l)1, regardless of the temperature or aw level tested. Total fumonisin production was higher at 0á98 aw and decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both the temperature or aw level tested. Total fumonisin production was higher at 0á98 aw and decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both and temperature (18 and 25°C) on a maize meal agar. Overall, PP was the antioxidant which was most effective at inhibiting strains of both species. The lag phase prior to growth and growth rates were signi®cantly decreased by PP and BHA at 10 and 20 mmol l)1, regardless of the temperature or aw level tested. Total fumonisin production was higher at 0á98 aw and decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both the temperature or aw level tested. Total fumonisin production was higher at 0á98 aw and decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both was most effective at inhibiting strains of both species. The lag phase prior to growth and growth rates were signi®cantly decreased by PP and BHA at 10 and 20 mmol l)1, regardless of the temperature or aw level tested. Total fumonisin production was higher at 0á98 aw and decreased by about 45±50% at 0á995 and 0á95 aw. Overall, BHT only inhibited fumonisin production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both production at 0á95 aw at 10 and 20 mmol l)1, while BHA was effective at most aw levels tested at 10 and 20 mmol l)1. Propylparaben completely inhibited fumonisin production by both