INVESTIGADORES
GIOVAMBATTISTA Guillermo
congresos y reuniones científicas
Título:
Association Study between DLA polymorphism and susceptibility to chronic superficial keratitis in German Shepherd dogs.
Autor/es:
IT V.; BARRIENTOS L. S.; ZAPATA G.; DÍAZ S; GIOVAMBATTISTA G.
Lugar:
Amterdam, Países Bajos
Reunión:
Conferencia; XXXI Conference of the International Society for Animal Genetics; 2008
Institución organizadora:
ISAG
Resumen:
Chronic Superficial Keratitis (CSK) is a progressive,
inflammatory and potentially blinding disease of the
canine bilateral cornea. The German shepherd is the
most commonly affected breed. CSK have been
described as an immune-mediated disease. The
increased MHC class II expression may play a part in
perpetuating the corneal inflammation seen in the
disease. Given the previously reported association
between the CSK and up-regulation of major
histocompatibility class II antigen expression, the
primary goal of this work is to determine the
association between the Dog Leukocyte Antigen
system (DLA) and the disease in German Shepherd
dogs. Blood samples were collected from twelve dogs
with pannus and twelve control dogs. The
polymorphism of DLA-DQA, -DQB and -DRB
promoters, and four DLA linked microsatellites was
genotyped. Genetic diversity was calculated for each
locus, and association between genetic markers and
presence/absence of pannus was evaluated.
Preliminary results showed that: (i) the allele DLADQA1-
DQA*p1a is fixed in both subpopulations; (ii)
two alleles of the DLA-DRB1 promoter (DLADRB1*
p1 and DLA-DRB1*p2) were detected with
similar gene frequencies in both groups; (iii) we have
only detect one (DLA-DQB*7) out of four DLA-DQB
alleles reported in German Shepherd, as well as, the
allele DLA-DQA*p1; (iv) three putative new DLADQB
promoter variants were detected in our sample;
(v) not significant differences were observed between
illness and control group for the microsatellites
FH2200 and FH2202; (vi) significant differences
between pannus and control for the loci FH2054 and
FH2975 (pFH2054 = 0.00744; pFH2975 =0.003214)
were observed; (vii) a relative risk of 6.25 and 2.75 for
the alleles FH2054*152 and FH2579*320,
respectively, were calculated; and (viii) an odds ratio
of 9 and 3.4 for these alleles, respectively, were
estimated.
perpetuating the corneal inflammation seen in the
disease. Given the previously reported association
between the CSK and up-regulation of major
histocompatibility class II antigen expression, the
primary goal of this work is to determine the
association between the Dog Leukocyte Antigen
system (DLA) and the disease in German Shepherd
dogs. Blood samples were collected from twelve dogs
with pannus and twelve control dogs. The
polymorphism of DLA-DQA, -DQB and -DRB
promoters, and four DLA linked microsatellites was
genotyped. Genetic diversity was calculated for each
locus, and association between genetic markers and
presence/absence of pannus was evaluated.
Preliminary results showed that: (i) the allele DLADQA1-
DQA*p1a is fixed in both subpopulations; (ii)
two alleles of the DLA-DRB1 promoter (DLADRB1*
p1 and DLA-DRB1*p2) were detected with
similar gene frequencies in both groups; (iii) we have
only detect one (DLA-DQB*7) out of four DLA-DQB
alleles reported in German Shepherd, as well as, the
allele DLA-DQA*p1; (iv) three putative new DLADQB
promoter variants were detected in our sample;
(v) not significant differences were observed between
illness and control group for the microsatellites
FH2200 and FH2202; (vi) significant differences
between pannus and control for the loci FH2054 and
FH2975 (pFH2054 = 0.00744; pFH2975 =0.003214)
were observed; (vii) a relative risk of 6.25 and 2.75 for
the alleles FH2054*152 and FH2579*320,
respectively, were calculated; and (viii) an odds ratio
of 9 and 3.4 for these alleles, respectively, were
estimated.
perpetuating the corneal inflammation seen in the
disease. Given the previously reported association
between the CSK and up-regulation of major
histocompatibility class II antigen expression, the
primary goal of this work is to determine the
association between the Dog Leukocyte Antigen
system (DLA) and the disease in German Shepherd
dogs. Blood samples were collected from twelve dogs
with pannus and twelve control dogs. The
polymorphism of DLA-DQA, -DQB and -DRB
promoters, and four DLA linked microsatellites was
genotyped. Genetic diversity was calculated for each
locus, and association between genetic markers and
presence/absence of pannus was evaluated.
Preliminary results showed that: (i) the allele DLADQA1-
DQA*p1a is fixed in both subpopulations; (ii)
two alleles of the DLA-DRB1 promoter (DLADRB1*
p1 and DLA-DRB1*p2) were detected with
similar gene frequencies in both groups; (iii) we have
only detect one (DLA-DQB*7) out of four DLA-DQB
alleles reported in German Shepherd, as well as, the
allele DLA-DQA*p1; (iv) three putative new DLADQB
promoter variants were detected in our sample;
(v) not significant differences were observed between
illness and control group for the microsatellites
FH2200 and FH2202; (vi) significant differences
between pannus and control for the loci FH2054 and
FH2975 (pFH2054 = 0.00744; pFH2975 =0.003214)
were observed; (vii) a relative risk of 6.25 and 2.75 for
the alleles FH2054*152 and FH2579*320,
respectively, were calculated; and (viii) an odds ratio
of 9 and 3.4 for these alleles, respectively, were
estimated.