Gene Frequency Distribution of the BoLA-DRB3 in the Dairy “Saavedreño” Creole Cattle

RIPOLI M. V.; LIRÓN J. P.; DE LUCA J. C.; ROJAS F.; DULOUT F. N.; GIOVAMBATTISTA G.

Biochemical Genetics

Kluwer

Año: 2004 vol. 42 p. 231 - 240

0006-2928

The objective of this study is to describe the gene frequency distribution of the bovine lymphocyte antigen (BoLA)-DRB3 locus in Saavedre˜no Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedre˜no Creole dairy cattle were genotyped for thelocus in Saavedre˜no Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedre˜no Creole dairy cattle were genotyped for the BoLA-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism. Twenty-two out of 53 previously identified BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles (BoLA-DRB3.2*7,allele by polymerase chain reaction and restriction fragment length polymorphism. Twenty-two out of 53 previously identified BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles (BoLA-DRB3.2*7,BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles (BoLA-DRB3.2*7,BoLA-DRB3.2*7, *8, *11, *16, *27, *36, and *37). The studied population exhibits a high degree of expected heterozygosity (he D 0:919). The FI S index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedre˜no Creole dairy cattle, with significant variation in allele frequency among cattle breeds., *11, *16, *27, *36, and *37). The studied population exhibits a high degree of expected heterozygosity (he D 0:919). The FI S index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedre˜no Creole dairy cattle, with significant variation in allele frequency among cattle breeds.he D 0:919). The FI S index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedre˜no Creole dairy cattle, with significant variation in allele frequency among cattle breeds.BoLA-DRB3.2 is a highly polymorphic locus in Saavedre˜no Creole dairy cattle, with significant variation in allele frequency among cattle breeds.