Haplotype determination of the upstream regulatory region and the second exon of the BoLA-DRB3 gene in Holstein cattle

GOSZCZYNSKI D.E.; RIPOLI M.V.; TAKESHIMA S-N; BALTIAN L.R.; AIDA Y.; GIOVAMBATTISTA G.

TISSUE ANTIGENS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2014 vol. 83 p. 180 - 183

0001-2815

Polymorphisms of the BoLA-DRB3 gene are located primarily in the second exon (antigen binding site) and, to a lesser extent, in the upstream regulatory region (URR). It can be hypothesised that exon 2 and the URR are under different types of natural selection. The aim of the present work was to determine the URR-exon 2 haplotypes; 34 Holstein samples were genotyped by direct sequencing. Seven URR alleles and twenty-three exon 2 alleles were detected, and three of the URR alleles were novel. Our results may suggest that no relationship exists between the URR and exon 2 of the BoLA-DRB3 gene (Linkage disequilibrium p value > 0.05), most likely due to recombination over time. Our results also suggest that both regions of class II genes may be included in the development of new genotyping methods based on next-generation DNA sequencing technologies.