Analysis of ELA-DRB exón 2 polymorphism by PCR-RFLP

PERAL-GARCÍA, P.; GOLIJOW, C.D.; GIOVAMBATTISTA, G.; DULOUT, F. N.; LOJO, M.M.

Journal of Equine Science

The Japanese Society of Equine Science

Año: 1999 vol. 10 p. 13 - 16

1340-3516

Most class II genes show a wide genetic variation both for the number of loci and the number of alleles among and within species. Sequence conservation along evolution has proven to be sufficiently high among domestic animals. Thus, primer sequences developed for one species could be employed to amplify sequences of another. The aim of this work was to develop a PCR-RFLP method for MHC DRB DNA typing in horses using bovine designed primers. The analysis of the ELA-DRB sequences revealed that all the eleven alleles previously described could be differentiated by the PCR-RFLP method using the restriction enzymes Hae III and Rsa I. The use of other restriction enzymes could be useful to recognize other variants, improving the accuracy of this DNA-based typing method.