Genetic structure of Varroa destructor populations infesting Apis mellifera colonies in Argentina

MAGGI , M;.; MEDICI, S; QUINTANA, M; RUFFINENGO,S; J.MARCANGELI,; GIMENEZ,P; S.FUSELLI,; EGUARAS M

EXPERIMENTAL AND APPLIED ACAROLOGY

SPRINGER

Lugar: Berlin; Año: 2012 vol. 55 p. 344 - 347

0168-8162

Although mitochondrial DNA mapping of Varroa destructor revealed the presence of several haplotypes, only two of them (Korean and Japanese haplotypes) werecapable to infest Apis mellifera populations. Even though the Korean haplotype is the only one that has been reported in Argentina, these conclusions were based on mites sampled in apiaries from a specific geographical place (Buenos Aires province). To study mites from several sites of Argentina could reveal the presence of the Japanese genotype, especially considering sites near to Brazil, where Japanese haplotype was already detected. The aim of this work was to study the genetic structure of V. destructor populations from apiaries located in various provinces of Argentina, in order to determine the presence of different haplotypes. The study was carried out between January 2006 and December 2009. Phoretic adult Varroa mites were collected from honey bee workers sampled from colonies of A. mellifera located in Entre Rı´os, Buenos Aires, Corrientes, Rı´o Negro, Santa Cruz and Neuque´n provinces. Twenty female mites from each sampling site were used to carry out the genetic analysis. For DNA extraction a nondestructive method was used. DNA sequences were compared to Korean haplotype (AF106899) and Japanese haplotype (AF106897). All DNA sequences obtained from mite populations sampled in Argentina,