Antiviral activity of quercetin loaded o/w microemulsion. Potential topical application.

VOLPE V; PEREZ SE; DOLCINI G; BUZZOLA F; GRUNHUT M

Congreso; 6ta Reunión Internacional de Ciencias Farmacéuticas (RICIFa 2020+1); 2021

Quercetin is a flavonoid compound that presents several therapeutic properties such as antioxidant, anti-inflammatory, antibacterial and antiviral, among others. However, the topical application of this compound is limited due to its poor solubility in water and its limited bioavailability.In this work it is proposed to use an O/W type microemulsion as a vehicle to load quercetin at different concentrations and evaluate its antiviral activity.For this, a microemulsion composed of isopropyl myristate (oil), tween 80/ethanol (surfactant/co-surfactant) and water was loaded with quercetin at differents concentrations that ranged from 50 to 200 mg/L-1. Subsequently, binding assays and viral titer reduction were performed using MDBK cells (Madin-Darby bovine kidney) and bovine alphaherpesvirus 1 (BoHV-1) Cooper strain (Titer: 106TCID50mL-1).The results showed a reduction of the viral titer and a reduction of the viral titer by means of viral adsorption test that varied from 38.3% to 70.7% and 45.6 to 74.2%, respectively, obtaining the best results when the concentration of quercetin loaded was 100 mg/L-1.In conclusion, a quercetin carrier system was obtained from biocompatible materials for its potential use as an antiviral.