Plant Cell Measurements with Mass Spectrometry for Speaking Cell Approach in Plant Growth Factories,

,YOUSEF GHOLIPOUR; H. NONAMI; ERRA-BALSELLS R.; ERRA-BALSELLS, ROSA; ERRA BALSELLS, ROSA

Milan

Congreso; 18th IFAC World Congress, (IFAC WC 2011), Milan, Italia.; 2011

International Federation Automatic Control - Bioscience Area

Plant Cell Measurements with Mass Spectrometry for Speaking Cell Approach  in Plant Growth Factories Hiroshi Nonami1, Yousef Gholipour1, Rosa Erra-Balsells2 1Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, Matsuyama, Japan 2CIHIDECAR-Departamento De Qímica Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Provided suitable MALDI matrixes are available to desorb/ionize favorite metabolites, contemporary MALDI Mass Spectrometry can analyze mixture of natural biomolecules with minimum preparation and high sensitivity. By applying this technique, it is possible to make a molecular profile of the sample (extract or tissue surface) which can provide insights to understand biochemical and physical status of plant during growth or unfavorable stress condition. Therefore, this technique is a great candidate to be an analytical tool for plant growth factories where fast and highly accurate analysis of metabolites can be quite helpful.  We have combined this technique with Pressure Probe to microsample living single cells and simultaneously measure pressure-related properties of single cells. Cell samples were deposited on selected matrixes followed by MALDI Mass Spectrometry analysis. Soluble sugars could be detected in very small volume of cytoplasm extract while verification of detected metabolites was also done. Additionally, we studied the applicability of some new materials as MALDI matrixes from metal and inorganic salts nanoparticles to carbon nanotubes and organi molecules for direct profiling of plant tissue. These matrixes show high selectivity for desorption/ionization of soluble sugars from the surface of plant tissue. Interestingly, profiled sugars in single cell samples were similar to those detected on the tissue surface. Accordingly, while our direct cell microsampling-analysis technique can provide cell-level chemical information, our direct tissue analysis technique can also easily produce a general profile of sugars and other metabolites contents of included cells.