Ethylenediaminetetraacetic acid (EDTA) as an auxiliary tool in the electrospray ionization mass spectrometry analysis of native and derivatized b-cyclodextrins, maltoses and fructans contaminated with Ca and/or Mg.

M.KANIZ FATEMA; HIROSHI NONAMI; S. L. GIUDICESSI; ERRA BALSELLS, ROSA

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2010 vol. 26 p. 1526 - 1529

1044-0305

Ethylenediaminetetraacetic acid (EDTA) as an auxiliary tool in the electrospray ionization mass spectrometry analysis of native and derivatized b-cyclodextrins, maltoses and fructans contaminated with Ca and/or Mg Silvana L. Giudicessi,1 M. Kaniz Fatema,2 Hiroshi Nonami2 and Rosa Erra-Balsells1* 1 CIHIDECAR-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428-Buenos Aires, Argentina. 2 Plant Biophysics/Biochem. Research Laboratory, Faculty of Agriculture, Ehime University, 790-8566-Matsuyama, Japan. Running title: EDTA in carbohydrate ESI-MS analysis Corresponding author: Rosa Erra-Balsells Departamento de Química Orgánica, FCEN, UBA, Ciudad Universitaria, 1428-Buenos Aires, Argentina  e-mail: erra@qo.fcen.uba.ar Te/Fax: +54-11-4576-3346 Abstract The effect of Ca2+ (and Mg2+) and the disodium salt of ethylenediaminetetraacetic acid (EDTA), a well known Ca2+ (and Mg2+) chelating agent, on the volatilization/ionization of carbohydrates by using electrospray ionization mass spectrometry has been studied. Model compounds such as maltoses (maltose to maltoheptaose), b-cyclodextrins (b-cyclodextrin, methyl-b-cyclodextrin, heptakis(2,6-di-O-methyl)-b-cyclodextrin, heptakis(2,3,6-tri-O-methyl)-b-cyclodextrin and 2-hydroxypropyl-b-cyclodextrin) and fructans (sucrose, 1-ketose, nystose and 1F-fructofuranosylnystose) were used.