Rapid characterization and nutrient status Halamphora luciae (Bacillariophyceae) using matrix-assisted ultravioleta laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)

YASMIN DAGLIOA, ; MARÍA LAURA SALUMA,; ROSA ERRA-BALSELLS; MARÍA CRISTINA MATULEWICZ

EUROPEAN JOURNAL OF PHYCOLOGY

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2018

0967-0262

In this study, the effects of N and P depletion on the production and structuralcharacterization of the cellular carbohydrate polymers of the estuarine diatom Halamphoraluciae in batch culture were examined using matrix-assisted laser desorption-ionizationtime-of flight mass spectrometry (MALDI-TOF MS) complemented with monosaccharidecomposition determination and structural analyses by methylation of aqueous extractedproduct. The MALDI MS analysis of the cells showed a similar profile in control and Nand P- depleted media, with a displacement to higher molecular weight for the grown indepleted media. When confronting with monosaccharide analyses, both nutrient depletionand culture aging led to an increment in glucose content, supporting the hypothesis thatMALDI-TOF MS in whole cells depicted the changes in chrysolaminarin. Thus,accumulation of the storage glucan was related with the displacement to increased relativeabundance of higher DP signals in aged and N- and P- deprived cultures of H. luciae.Methylation analysis confirmed the presence of chrysolaminarin, a ?À-(1?¨3) linked glucanwith branching in C2 and C6, where the glucan backbone presented substitution every fourglucose residues. The ?À-(1?¨3)-D-glucan was also detected in the cingules by fluorescencewith aniline blue.394041 Keywords: Cellular carbohydrate polymers, Diatom cells, Halamphora luciae, MALDI42TOF MS, Nutrient stress.43Page 3 of 43URL: http:/mc.manuscriptcentral.com/tejp Email: ejp@nhm.ac.ukEuropean Journal of Phycology1234567891011121314151617181920212223242526272829303132333435363738394041424344