Detection of Protein from Detergent Solutions by Probe Electrospray Ionization Mass Spectrometry

M. K. MANDAL; LEE CHUIN CHEN; ZHAN YU; H. NONAMI,; K. HIRAOKA; ERRA BALSELLS, ROSA

JOURNAL OF MASS SPECTROMETRY

JOHN WILEY & SONS LTD

Lugar: Londres; Año: 2011 vol. 46 p. 967 - 975

1076-5174

Abstract: Detergents are necessarily used for different extraction protocols of proteins from biological cells or tissues.  After the extraction, elimination of detergent is necessary for the better performance of ESI/MS. Elimination of detergents is laborious and time-consuming and also sample loss may be unavoidable.  Probe electrospray ionization (PESI) developed in our laboratory has been found to be tolerant to the presence of salts and buffers in sample solutions. In this report, it was examined whether PESI is applicable to the sample solutions that contain high-concentration detergents. It was found that PESI is highly tolerant to the presence of sodium dodecyl sulphate (SDS), cetyl trimethylamminium bromide (CTAB), Triton X100, and 3-[3-chlolamidopropyl] dimethylammonio]-1-propanesulfonate (CHAPS) compared with conventional ESI and nanoESI. Therefore, PESI can be a potential analytical tool for direct analysis of protein extracts and digests containing high-concentration detergents.