Transcriptome analysis reveals novel genes involved in nonhost response to bacterial infection in tobacco

DAURELIO, L.D; PETROCELLI, S.; BLANCO, F.; HOLUIGUE, L.; OTTADO, J.; ORELLANO, E.G.

JOURNAL OF PLANT PHYSIOLOGY

ELSEVIER GMBH

Lugar: Leipzig; Año: 2011 vol. 168 p. 382 - 391

0176-1617

Plants are continuously exposed to pathogen challenge. The most common defense response to pathogenic microorganisms is the non host response, which is usually accompanied by transcriptional changes. In order to identify genes involved in non host resistance, we evaluated the tobacco transcriptome profile after infection with Xanthomonas axonopodis pv. citri (Xac), a non host phytopathogenic bacterium. cDNA-amplified fragment length polymorphism was used to identify differentially expressed transcripts in tobacco leaves infected with Xac at 2, 8 and 24h post-inoculation. From a total of 2087 transcript-derived fragments (TDFs) screened (approximately 20% of the tobacco transcriptome), 316 TDFs showed differential expression. Based on sequence similarities, 82 differential TDFs were identified and assigned to different functional categories: 56 displayed homology to genes with known functions, 12 to proteins with unknown functions and 14 did not have a match. Real-time PCR was carried out with selected transcripts to confirm the expression pattern obtained. The results reveal novel genes associated with non host resistance in plant–pathogen interaction in tobacco. These novel genes could be included in future strategies of molecular breeding for non host disease resistance.