Cloning and Production of Thermostable Enzymes for the Hydrolysis of Steryl Glucosides in Biodiesel

PEIRU, SALVADOR; MENZELLA, HUGO G.; AGUIRRE, ANDRES; CASTELLI, MARIA EUGENIA; RASIA, RODOLFO M

METHODS IN MOLECULAR BIOLOGY (CLIFTON, N.J.)

Humana Press Inc.

Año: 2021 vol. 2290 p. 203 - 214

1064-3745

Vegetable oil-derived biodiesels have a major quality problem due to the presence of precipitates formed by steryl glucosides, which clog filters and injectors of diesel engines. An efficient, scalable, and cost-effective method to hydrolyze steryl glucosides using thermostable enzymes has been developed. Here, methods to discover, express in recombinant microorganisms and manufacture enzymes with SGase activity, as well as methods to treat biodiesel with such enzymes, and to measure the content of steryl glucosides in biodiesel samples are presented.