Magneto-Targeting of adipose-derived stem cells (AdSC) as periphrial nerve regeneration strategy.

P. A. SOTO; G. PIÑERO; P. SETTON-AVRUJ; V. USACH; D. F. CORAL; M.B. FERNÁNDEZ VAN RAAP; A. ROIG

Buenos Aires

Encuentro; Reunion Conjunta de Sociedades de Biociencias; 2017

Neuropathies are common problems in public health with high prevalence worldwide. Despite the regenerative capability of the peripheral nervous system (PNS), the poor clinical evolution of patients turns these affections into a crippling disease, which is why the development of new regenerative therapies is of great importance.Research in stem cells has recently become an important tool to accelerate regeneration in the PNS and adipose-derived stem cells (AdSC) are a promising tool to develop regenerative therapies.The aim of the present work was to test whether magnetic nanoparticle targeting of AdSC (AdSC-MNP) can enhance the regenerative ability of AdSC upon rat sciatic nerve crush.To this end, AdSC were harvested from 60-day-old Wistar rats andcharacterized for multipotent cell marker expression.MNP were synthetized by co-precipitation and characterized by vibrating sample magnetometry, transmission electron microscopy, electron diffraction and nanoparticle tracking analysis. MNP internalization was evaluated through dcmagnetometry experiments and AdSC-MNPviability was studied through MTT assays.The effect of transplanted AdSC-MNP was assessedthrough western blot and immunofluorescence analysis.AdSC-MNPwere positive for CD90 and CD105 and MTT assays showed MNP not to affectcell viability. In addition, VSM and epifluorescence microscopy showed the arrival of transplanted AdSC-MNP exclusively at the injured nerve.Furthermore, Western blot and immunofluorescence for MBP demonstrated AdSC-MNP beneficial effects on nerve regeneration.Our results prove magneto targeting to facilitateAdSC arrival at the lesion site, probably enhancing nerve regeneration. Further experiments will determine the effect of AdSC-MNP on other aspects of the regeneration process.Keywords: cell therapy, magnetic nanoparticles,neuroregeneration, magneto targeting.