In vivo evaluation of the antioxidant and photoprotective capacity of Gadusol and Mycosporine like amino acids in the stratum corneum using Confocal Raman spectroscopy

MAIRA GASPAR TOSATO; DALILA ORALLO; MARIA SANDRA CHURIO; AIRTON ABRAHÃO MARTIN; LELIA DICELIO

Cordoba

Congreso; 16th International Congress on Photobiology; 2014

Chronic exposure to ultraviolet A (UVA) (320-400 nm) can cause premature aging and cancer of the skin inducing the production of reactive oxygen species (ROS) [1]. Recent studies have focused on marine organisms as a source of natural bioactive molecules with therapeutic properties. Mycosporine like amino acids (MAA) porphyra-334 and shinorine and gadusol (Gad) were respectively isolated from marine red algae and fish roes. We investigated the effects of polymers gels containing Pluronic ® F-127 as in vivo delivery systems of MAA and Gad in human skin [2][3]. A total of 5 healthy volunteers (females) aged 20-30 and skin type II participated in this study. The areas of forearm were treated with formulations during 3 consecutive days. UV exposure was using lamp xenon. In vivo Raman measurements were performed in the fingerprint region (400?1800 cm−1) using a 785 nm laser. Raman spectra in vivo were collected for all treatments and control sites at different depths below the skin surface until 56 μm depth (Figure 1). The depth profiles of the trans-isomer of urocanic acid (t-UCA) amount in the stratum corneum as a function of the treatment are shown in Figure 2. The amount of t-UCA decreased immediately after UV exposure and increased by the application of gel on the skin surface.