INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
Facile Glycoenzyme Wiring to Electrode Supports by Redox-Active Biosupramolecular Glue
DIEGO PALLAROLA; N. QUERALTO; W. KNOLL; OMAR AZZARONI; FERNANDO BATTAGLINI
CHEMISTRY-A EUROPEAN JOURNAL
WILEY-V C H VERLAG GMBH
Lugar: Weinheim, Germany; Año: 2010 vol. 16 p. 13970 - 13970
The integration of redox glycoproteins on conductive supports plays a pivotal role in the successful design of bioelectronic platforms, including, among others, biosensors or biofuel cells.This is strongly related to the ability to control the interfacial architecture and the quality of the associationbetween the enzyme and the electrode surface. This aspect is particularly important for amperometric biosensors by virtue of the complex interplay between the reactions thatgive rise to the electronic signal and the assemblage of the biorecognition elements onto the solid support. AparT from retaining the biological activity of the enzyme, the immobilisationprocedure must guarantee the accessibility of its active site to the target analyte and other molecules involved in the biorecognition event. It is known that many immobilization methodologies can induce conformational changes in the enzyme, which could be accompanied by asignificant loss of enzymatic activity.