INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
Supramolecular assembly of glucose oxidase on concanavalin Amodified gold electrodes
DIEGO PALLAROLA; NURIA QUERALTO; FERNANDO BATTAGLINI; OMAR AZZARONI
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
ROYAL SOC CHEMISTRY
Lugar: London; Año: 2010 vol. 12 p. 8071 - 8071
There is a growing quest for the construction of functional supramolecular architectures toefficiently translate (bio)chemical events into easily measurable signals. This interest originates fromits inherent scientific relevance as well as from their potential applications in the ever-flourishingareas of bioelectronics and biosensing. Herein, we describe the immobilization of glycoproteinsonto electrode surfaces based on recognition-mediated supramolecular processes. Quartz crystalmicrobalance with dissipation (QCM-D), surface plasmon resonance (SPR) spectroscopy, andelectrochemical (EC) measurements were used to characterize the structural and functional featuresof these bio-supramolecular systems. Carbohydratelectin interactions were successfully used tobuild up stable assemblies of glucose oxidase (GOx) layers mediated by the recognition propertiesof concanavalin A supramolecular architectures. The catalytic response of GOx indicates that thewhole population of enzymes incorporated in the supramolecular architecture is fully active. Eventhough lectincarbohydrate interactions are rather weak, the multivalency effects prevailing in thesupramolecular assembly confer remarkable stability to the interfacial architecture, thus preventingthe release of the enzyme from the surface even with high glucose (ligand) concentrations. Thisapproach represents a simple and straightforward route to locally address functional glycoproteinsat interfaces. In this context, we consider that the versatility of a supramolecular assembly usingbiological interactions could open up new ways of envisioning or to generate new ideas for thefuture development of highly efficient bioelectronic platforms.