INQUIMAE 12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
Autor/es:
ROBERTO ARAYA; VICTORIA ANDINO PAVLOVSKY; RAFAEL YUSTE; ROBERTO ETCHENIQUE
Revista:
ACS Chemical Neuroscience
Editorial:
American Chemical Society
Referencias:
Año: 2013 p. 1163 - 1167
ISSN:
1948-7193
Resumen:
We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.
