INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
Sol?gel silica platforms for microalgae-based optical biosensors
ANA MERCEDES PERULLINI; FERRO, YANNIS; CLAUDE DURRIEU; MATIAS JOBBAGY; ALDABE BILMES, SARA
JOURNAL OF BIOTECHNOLOGY
ELSEVIER SCIENCE BV
Lugar: Amsterdam; Año: 2014 vol. 179 p. 65 - 65
An advanced hybrid biosensing platform with improved optical quality is developed based on the acidic encapsulation of microalgi in silica matrices synthesized by TAFR (tetraethoxysilane derived alcohol free route). The three microalgi (Chlorella vulgaris, Pseudokirchneriella subcapitata and Chlamydomonas reinhardtii) were previously immobilized in alginate following the two-step procedure. Tuning the alginate protecting function with the aid of Tris?HCl buffer, the sol?gel synthesis was conducted at pH 4.0 well below the tolerance limit imposed by the encapsulated microalgae. The acidic condensation of Si(IV) generates silica matrices with outstanding optical properties that suit the requirements of biosensors based on optical detection methods.