INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
Phosphate Mediated Adsorption and Electron Transfer of Cytochrome c. A Time-Resolved SERR Spectroelectrochemical Study.
CAPDEVILA, D.A.; MARMISOLLÉ, W.A.; WILLIAMS, F.J.; MURGIDA, D.H.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
ROYAL SOC CHEMISTRY
Lugar: CAMBRIDGE; Año: 2012 vol. 15 p. 5386 - 5386
The study of proteins immobilized on biomimetic or biocompatible electrodes represents an active field of research as it pursues both fundamental and technological interests. In this context, adsorption and redox properties of cytochrome c (Cyt) on different electrode surfaces have been extensively reported,10 although in some cases with contradictory results. Here we report a SERR spectroelectrochemical study of the adsorption and electron transfer behaviour of the basic protein Cyt on electrodes coated with amino-terminated monolayers. The obtained results show that inorganic phosphate (Pi) and ATP anions are able to mediate high affinity binding of the protein with preservation of the native structure and rendering an average orientation that guaranties efficient pathways for direct electron transfer. These 15 findings aid to the design of Cyt-based bioelectronic devices and to understanding the modulation by Pi and ATP of physiological functions of Cyt.