INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
Modulating Surface Density of Proteins via Caged Surfaces and Controlled Light Exposure
MARTA ALVAREZ; JOSÉ MARÍA ALONSO; OSCAR FILEVICH; MANIRAJ BHAGAWATI; ROBERTO ETCHENIQUE; JACOB PIEHLER; ARÁNZAZU DEL CAMPO
AMER CHEMICAL SOC
Lugar: Washington D.C.; Año: 2011 vol. 27 p. 2789 - 2789
We demonstrate the possibility of tuning the degree of functionalization of a surface using photoactivatable chemistries and controlled light exposure. A photosensitive organosilane with a protected amine terminal group and a tetraethyleneglycol spacer was synthesized. A o-nitrobenzyl cage was used as the photoremovable group to cage the amine functionality. Surfaces with phototunable amine densities were generated by controlled irradiation of silica substrates modiﬁed with the photosensitive anchor. Protein layers with diﬀerent densities could be obtained by successive coupling and assembly steps. Protein surface concentrations were quantiﬁed by reﬂectance interference. Our results demonstrate that the protein density correlates with the photogenerated ligand density. The density control was proved over four coupling steps (biotin, SAv,BTtris-NTA, MBP, or GFP), indicating that the interactions between underlying layer and soluble targets are highly speciﬁc and the immobilized targets at the four levels maintain their full functionality. Protein micropatterns with a gradient of protein density were also obtained.