INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Fast two-photon neuronal imaging and control using a spatial light modulator and ruthenium compounds
Autor/es:
PETERKA, DARCY S; NIKOLENKO, VOLODYMYR; FINO, ELODIE; ARAYA, ROBERTO; ETCHENIQUE, ROBERTO; YUSTE, RAFAEL
Revista:
SPIE
Editorial:
SPIE-INT SOC OPTICAL ENGINEERING
Referencias:
Lugar: BELLINGHAM; Año: 2010 vol. 7548 p. 754841 - 754841
ISSN:
0277-786X
Resumen:
We have developed a spatial light modulator (SLM) based microscope that uses diffraction to shape the incoming two-photon laser source to any arbitrary light pattern. This allows the simultaneous imaging or photostimulation of different regions of a sample with three-dimensional precision at high frame rates. Additionally, we have combined this microscope with a new class of two photon active neuromodulators with Ruthenium BiPyridine (RuBi) based cages that offer great flexibility for neuronal control.