IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EGF-dependent bcl-X expression in mammary epithelial cells
Autor/es:
GRINMAN, D; ROMORINI, L.; COSO, O.A.; PECCI, A.
Lugar:
Los Cocos, Cordoba
Reunión:
Workshop; SISTAM 2010; 2010
Resumen:
EGF activates signaling pathways asociated with celular
proliferation and apoptosis. We have previously shown that the expression of
the antiapoptotic protein Bcl-XL is regulated by EGF throughout apoptosis
inhibition in HC11 mammary epithelial cells, being PI3K/AKT the main pathway
involved in this process. EGF mediated bcl-X induction occurs mainly through
the activation of bcl-X promoter P1, which contains CREB and ETS response
elements. In this work we analyzed the possible participation of these
transcription factors on EGF dependent bcl-X expression. HC11 cells where
co-transfected with a pP1-Luc reporter vector together with pAKT and the above
mentioned transcription factors expression vectors. Results showed that CREB
induced bcl-X P1activation response (3,5±0,2 folds). Co-transfeccions of P1-Luc
and a CREB dominant negative expression vector confirmed P1 as a CREB target.
Moreover, EGF-dependent CREB phosphorylation was also observed. Conclusions:
PI3K/AKT activation by EGF increases bcl-X levels by regulating P1, being CREB
a potential transcription factor mediating this effect.