Long-term fetal developmental alterations and cardiopathy associated with oxidative stress induced after perigestational alcohol consumption up to organogenesis, in mouse

VENTUREIRA, MR; CEBRAL, E.; GUALDONI G; ELIA EVELIN

Cordoba

Congreso; IXI LASBRA International Meeting; 2020

Previously, perigestational moderate alcohol consumption up to organogenesis (day 10 of mouse gestation (D10), produces abnormal embryos with high apoptosis, diminished proliferation and altered myocardial embryonic wall. The present study assessed the effects of perigestational alcohol intake up to D10 and alcohol deprivation up to D13, on fetal development, cardiac histology, ultrastructure, proliferation and apoptosis and oxidative status. Ethanol 10% in drinking water was administered to murine CF-1 females for 15 days before and up to D10, and gestation continued with water until D13 (treated females (TF)). Control females (CF) were administered with drinking water without ethanol. TF consumed 18 g/kg/day, resulting in 25% EDC. Perigestational alcohol administration up to D10 did not affect differentiation and growth of D13-fetuses, but produced high frequency of abnormal fetuses compared to CF (27% vs 3.5 %, p