STUDY OF THE JOINT ACTION OF STEROID HORMONE RECEPTORS IN THE MECHANISMS THAT CONTROL INFLAMMATORY RESPONSE

OGARA M.F.; NOTO M; PECCI A .

Bariloche

Simposio; SISTAM 2018; 2018

Inflammation is a defense mechanism against infections or damaged tissue. While this response is active, homeostatic processes that balance pro- and anti-inflammatory signaling pathways are induced. If unbalanced, these control mechanisms lead to deregulated inflammation. Studies about inflammatory response allowed the development of therapies against several disorders, and glucocorticoids have been widely used as immunosupressors and anti-inflammatory drugs. However, other steroid ligands and their receptors (e.g. LXRs) have also been proved to modulate the inflammatory response. Steroid receptors share structural and functional properties and despite the cross action found between some of them in several cellular responses, there are no studies that assess whether GR and LXRs functional interact in immune cells. We use the pro-monocytic human cell line U937, which can be differentiated into macrophage-like cells in the presence of phorbol myristate acetate (PMA). This treatment stimulates the expression of several pro-inflammatory genes such as CD11b, IL6, IL1B, TNFα and COX2. The presence of LXRs agonist GW3965 does not seem to affect their expression whereas the synthetic glucocorticoid dexamethasone (Dex) inhibits PMA-induction of these markers (p