Runx1 gene expression regulation in triple negative breast cancer

SOFIA MARIA SOSA; NATALIA RUBINSTEIN; VIRGINIA DANSEY; SANTIAGO RODRIGUEZ SEGUI

CABA

Congreso; Reunion Conjunta de Sociedades de Biociencia; 2017

Triple negative breast cancer (TNBC) is a heterogeneous group of diseases associated with early recurrence and low survival rates. Treatment options are limited due to the lack of specific therapeutic targets and are consequently managed with standard chemotherapy. Additionally, TNBC is associated with epithelial-mesenchymal transition (EMT). EMT is considered a process implicated in the metastatic cascade involving cellular conversion toward an invasive cell type and recent evidences strongly suggest that EMT process might be involved in tumor chemoresistance. It was found that RUNX1 protein expression correlates with poor prognosis in TNBC patients. Results from our group demonstrate that Runx1 protein is able to up regulate the expression of RSPO3 (oncogene) and down regulate GJA1 (tumor suppressor gene) on TNBC cell lines. We demonstrate that Runx1 is necessary for mammary tumor cell migration. Our main goal was to evaluate how Runx1 gene expression is regulated in TNBC. Here we show that Runx1 gene expression and transcriptional activity is significantly up regulated in Py2T after TGF treatment (p