Angiotensin-(1-7) counteracts the transforming effects triggered by angiotensin II in breast cancer cells.

SUBERBORDES MV; SCHERE LEVY C.; KORDON EC; CAMBADOS N.

Buenos Aires

Congreso; Reunión Conjunta Sociedades Biociencias; 2017

SAIC

Angiotensin (Ang) II, the main effector peptide of the renin-angiotensin system, has been implicated in multiple aspects of cancer progression such as proliferation, migration, invasion, angiogenesis and metastasis. Ang-(1-7), is a biologically active heptapeptide, gen- erated predominantly from AngII by the enzymatic activity of angio- tensin converting enzyme 2 (ACE 2). Previous studies have shown that Ang-(1-7) counterbalances AngII actions in different pathophysiological settings. In this study, our aim was to analysed the impact of Ang-(1-7) on AngII-induced pro-tumorigenic features on breast can- cer cells (MDA-MB-231). We found that Ang-(1-7) abrogated AngII induced migration and invasion of the MDA-MB-231 cells as well as pro-angiogenic events such as the stimulation of MMP-9 activity and VEGF expression. In addition, similar to Ang-(1-7), the treat- ment of cells with an anti-angiogenic VEGF antibody (bevacizum- ab), abolished AngII ?induced cell migration of MDA-MB-231 cells. Together, these results demonstrate for the first time that Ang-(1-7) counteracts tumor aggressive signals stimulated by AngII in breast cancer cells.