IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ROLE OF CHROMATIN IN THE COUPLING BETWEEN TRANSCRIPTION AND ATERNATIVE SPLICING
Autor/es:
SCHOR IE; RASCOVAN N; PELISCH F; ALLÓ M; FIZBEIN A; KORNBLIHTT AR
Lugar:
Cold Spring Harbor, NY, USA
Reunión:
Congreso; CSH Eukaryotic mRNA Processing Meeting; 2009
Institución organizadora:
Cold Spring Harbor Laboratory (CSHL)
Resumen:
<!--
/* Font Definitions */
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;
mso-font-charset:0;
mso-generic-font-family:roman;
mso-font-pitch:variable;
mso-font-signature:-1610611985 1107304683 0 0 159 0;}
@font-face
{font-family:Palatino;
mso-font-alt:"Book Antiqua";
mso-font-charset:0;
mso-generic-font-family:auto;
mso-font-pitch:variable;
mso-font-signature:50331648 0 0 0 1 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-unhide:no;
mso-style-qformat:yes;
mso-style-parent:"";
margin:0cm;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:"Times New Roman","serif";
mso-fareast-font-family:"Times New Roman";
mso-ansi-language:ES-TRAD;
mso-fareast-language:ES-TRAD;}
p.MsoBodyText3, li.MsoBodyText3, div.MsoBodyText3
{mso-style-unhide:no;
mso-style-link:"Texto independiente 3 Car";
margin-top:0cm;
margin-right:0cm;
margin-bottom:6.0pt;
margin-left:0cm;
mso-pagination:widow-orphan;
font-size:8.0pt;
font-family:"Times New Roman","serif";
mso-fareast-font-family:"Times New Roman";
mso-ansi-language:ES-TRAD;
mso-fareast-language:ES-TRAD;}
span.Textoindependiente3Car
{mso-style-name:"Texto independiente 3 Car";
mso-style-unhide:no;
mso-style-locked:yes;
mso-style-link:"Texto independiente 3";
mso-ansi-font-size:8.0pt;
mso-bidi-font-size:8.0pt;
mso-ansi-language:ES-TRAD;
mso-fareast-language:ES-TRAD;}
.MsoChpDefault
{mso-style-type:export-only;
mso-default-props:yes;
font-size:10.0pt;
mso-ansi-font-size:10.0pt;
mso-bidi-font-size:10.0pt;}
@page Section1
{size:612.0pt 792.0pt;
margin:70.85pt 3.0cm 70.85pt 3.0cm;
mso-header-margin:36.0pt;
mso-footer-margin:36.0pt;
mso-paper-source:0;}
div.Section1
{page:Section1;}
-->
Changes in RNA polymerase II (pol II) elongation rates
modulate alternative splicing choices, affecting the timing at which nascent
pre-mRNA splice sites and regulatory sequences are presented to the spliceosome
(an effect known as kinetic coupling). One of the ways in which this situation
could be relevant in regulation of endogenous alternative splicing choices is
by dynamic changes in intragenic chromatin structure that could in turn affect
RNA processivity or elongation rate. Using the exon 18 (E18) of the NCAM gene
as a model we have determined that intragenic histone acetylation, triggered by
membrane depolarization of N2a cells, decreases exon inclusion. This treatment
also causes a more relaxed chromatin conformation in the E18 region.
Accordingly, inhibition of DNA methylation by 5-aza-citidine can reduce the
differentiation- induced enhancement of E18 inclusion, further suggesting that
chromatin relaxation is associated to alternative exon skipping. Analysis of
the participation of different histone acetyl transferases (HATs) in
alternative splicing regulation show that CBP/p300 is implicated in the
regulation of E18 splicing in response to depolarization. These results raise
the possibility that balance between different histone modifications could
serve as a point of control of transcriptionally-coupled alternative splicing,
and that this balance can be modulated in different physiological situations.