Early placental angiogenesis-vascularization and VEGF/KDR receptor expression during mouse organogenesis after perigestational alcohol consumption

VENTUREIRA MR; SOBARZO CMA; NAITO M; ZANUZZI C; BARBEITO C; CEBRAL E

Mar del Plata

Congreso; VI Latin American Symposium on Maternal-Fetal Interaction and Placenta V Latin American Symposium on Reproductive Immunology; 2015

SLIMP - Latin American Society for Maternal Fetal Interaction and Placenta

The normal growth of the fetus depends on adequate placental development, in which the expression of trophoblast-decidual VEGF (vascular endothelial growth factor) conducts angiogenesis-vascularization. Previously, we found that perigestational alcohol consumption at mouse organogenesis (day 10 of gestation), causes embryo resorption, reduces decidualization and leads to histomorphological alterations of the decidual tissue.Objective: The aim was to study, after periconceptional alcohol intake, vascularization of early placentation by monitoring the number of uNK, VEGF and KDR-receptor expressions as well as KDR-activation, in murine decidua and labyrinth during organogenesis.Methods: Ethanol 10% was administered in the drinking water to CF-1 murine females (TF) for 17 days before and up to day 10 of gestation; water was administered to control females (CF). Labyrinthine development and decidual (De) vascularization (H-E, histochemistry for BSI-lectin, morphometry of decidual vascular area and labyrinth), the presence and number of uNKs (PAS and DBA-lectin) and the expression of VEGF and KDR (total and phosphorylated form) by immunohistochemistry, immunofluorescence-confocal and western blot (WB) were analyzed in the implantation sites (IS).Results: Increased percentage of TF-IS had poor labyrinthine growth and reduced De-vascular lumen (p