Synaptic NF-kappa B localization dynamics

A. SALLES; M. LAGOS; M. BOCCIA; M. BLAKE; N. CORBI; C. PASSANATI; C. M. BARATTI; A. ROMANO; R. FREUDENTHAL

Buenos Aires

Congreso; ISN Special Conference 2012 ?Synapses and dendritic spines in health and disease? Buenos Aires, September 12-15,2012; 2012

ISN

results showed that the transcription factor is also present in synaptic terminals. More importantly we found NF-kappa B strongly bound to membranes of these terminals. Another pool of NF-kappa B has been found in the synaptosomal content, and its activation measured through Electro Mobility Shift Assays (EMSA) is modified during consolidation of shocked mice, as compared with naı¨ve and unshocked mice. The membrane-bound NF-kappa B increases 5 min post training only in shocked mice. The relocalization of the transcription factor during learning could implicate a new function of NF-kappa B that might intervene in the consolidation process. The aim of this research is to study the dynamics of synaptic NF-kappa B activation during consolidation, discussing the novel localization of the transcription factor in membranes of synaptic terminals.