Effect of repetitive cocaine ?binge? administration on GAD65/67 and T- type subunits levels from GABAergic somatosensory thalamic neurons in mice?

GOITIA BELEN; RAINERI MARIANA; GONZALEZ LAURA E.; ROZAS JOSE L.; UCHITEL OSVALDO D.; D., RILL- GARCIA EDGAR; BISAGNO VERONICA; URBANO FRANCISCO J.

Cordoba - Huerta Grande

Congreso; XXVI Congreso anual SAN; 2011

A cocaine ?binge? induces an enhancement in both GABAergic transmission and T-type calcium currents on Ventrobasal (VB) thalamic neurons, which were prevented by the systemic administration of T-type calcium channel antagonists mibefradil and 2-octanol. However, it is unknown if protein levels of GAD65/67 or T-type calcium channel subunits are affected by cocaine. The objective of this study 230 # was to assess, using Western Blotting, the effects of acute and chronic cocaine ?binge? on GAD65/67, Cav3.1 and Cav3.3 T-type subunits protein levels compared to saline administration from mice thalamic VB and Reticular (RetN) nuclei. One day after a chronic ?binge? protocol (one ?binge? a day, for 3 days) an increase in protein levels of Cav 3.1 was observed in the VB nucleus (ANOVA p=0.01), while no changes in GAD65/67 levels were found. After a fourth ?binge? administration, no changes were observed in VB Cav 3.1 protein levels. GAD67 levels were increased in RetN nucleus (ANOVA p=0.024). No differences were found in GAD65/67 protein levels in the VB nucleus. Changes in the expression levels of these two key proteins may help understand alterations in thalamocortical networks of long-term cocaine abusers. Grants: PICT 2007-1009/2008-2019, PIDRI-PRH 2007, PIP 11420100100072. was to assess, using Western Blotting, the effects of acute and chronic cocaine ?binge? on GAD65/67, Cav3.1 and Cav3.3 T-type subunits protein levels compared to saline administration from mice thalamic VB and Reticular (RetN) nuclei. One day after a chronic ?binge? protocol (one ?binge? a day, for 3 days) an increase in protein levels of Cav 3.1 was observed in the VB nucleus (ANOVA p=0.01), while no changes in GAD65/67 levels were found. After a fourth ?binge? administration, no changes were observed in VB Cav 3.1 protein levels. GAD67 levels were increased in RetN nucleus (ANOVA p=0.024). No differences were found in GAD65/67 protein levels in the VB nucleus. Changes in the expression levels of these two key proteins may help understand alterations in thalamocortical networks of long-term cocaine abusers. Grants: PICT 2007-1009/2008-2019, PIDRI-PRH 2007, PIP 11420100100072.