IFIBYNE   05513
INSTITUTO DE FISIOLOGIA, BIOLOGIA MOLECULAR Y NEUROCIENCIAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
5-Brtomo-2' deoxyuridine is toxic to olfactory epithelium cells at concentrations widely used for studies of cell lineage
Autor/es:
RAICES, M.; JUNGBLUT, L.; PAZ DA; POZZI, AG
Lugar:
Huerta Grande, Córdoba
Reunión:
Congreso; XXVI Congreso de la SAN; 2011
Institución organizadora:
Sociedad Argentina de Neurociencia
Resumen:
The 5-bromo-2'-deoxyuridine (BrdU),
is a thymidine analogue that incorporates in to the DNA of dividing cells
during the S-phase of the cell cycle. This analogue is widely used for the
study of mitosis and cell lineage in vitro and in vivo. However, there are
reports that questioned the validity of its use in some biological systems
where BrdU showed cellular toxicity. The current work examines the effect of BrdU
incorporation on the phenotype of cells in the olfactory epithelium (OE). We
found a decrease in mature olfactory receptor neurons (ORNs) as a function of
BrdU exposition time when we used 10 mM BrdU. We also observed an increase in
the number of apoptotic cells after the BrdU administration. Moreover, while
the epithelial structure in BrdU treated animals was maintained, the
histochemical analysis showed alterations in secretory vesicles from the
sustentacular cells. BrdU 0,1-1 mM did not show the same toxic effects. We
conclude that the use of BrdU 10 mM (doses of current use in the literature)
has toxic effects on ORNs, activating classical cell death pathways and
altering the histomorphology of the sustentacular cells. These results
demonstrate the importance of using the correct dose of BrdU in neurogenesis
studies both in vivo and in vitro.