CONICET | Buscador de Institutos y Recursos Humanos

@font-face { font-family: "Times New Roman"; }@font-face { font-family: "Comic Sans MS"; }p.MsoNormal, li.MsoNormal, div.MsoNormal { margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman"; }table.MsoNormalTable { font-size: 10pt; font-family: "Times New Roman"; }div.Section1 { page: Section1; } STRATEGIES TOWARD IDENTIFYING THE SOURCE OF ENDOGENOUS 5-HT INVOLVED IN THE SSRI EFFECT M. A. Calviño Laboratorio de Fisiología y Biología Molecular. FCEyN (UBA). IFIBYNE (CONICET). Selective serotonin reuptake inhibitors (SSRI) evoke synaptic activity in identified neurons from leech ganglia, through the activation of a cord spanning interneuron. We asked about the source of endogenous 5-HT involved in this effect. Local and remote Retzius neurons and interneurons 21/61 are possible candidates (IIRNC2010). To answer the question we used two strategies: 1) to inhibit the activity of the candidates and block the effect, and/or 2) to stimulate the activity of the candidates and reproduce the effect. Based on the connectivity of the serotonergic neurons in the leech ganglia, hyperpolarizing (-10 nA) one of them inhibits the rest. However, we did not know if a hyperpolarizing current applied to a serotonergic soma in a ganglion arrives to the terminal that innervates the adjacent ganglion without decay, and thus blocks the release of 5-HT. Thus, we studied if applying a hyperpolarizing current (-10 nA) in an S cell affects the excitability of the S cell from an adjacent ganglion, taking into account that they are both connected by an electrical synapse. Results shown that the signal decay with distance (n = 3) and therefore it is not a valid strategy. The stimulation of serotonergic neurons by different protocols has not been successful (rate of success = 3/16).